Info-enriched characterization of mobile populations. Morphological info from photos can add into the richness of flow cytometry information. As an example, the determine exhibits an normally typical apoptosis assay employing Annexin V and PI, incorporating cell imaging to characterize cells in each population to reveal morphologically distinctive options. These insights could not have been obtained from multiplex staining on your own.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-pace brightfield digital camera captures and merchants images of detected activities at a charge around six,000 photos for each 2nd, dependant upon the flow amount and picture dimensions. For increased overall flexibility, Attune Cytometric Software package helps you to adjust the image capture frequency as wanted and select a particular gate for impression capture, if demanded.
(B) Uncooked 264.seven macrophages will often be annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The amount of singlets obtainable for analysis raises when the consumer trains and employs a far more precise design.
Find new insights into your experimental information by visualizing populations utilizing combined fluorescence and morphology data. Our software program interface wizards empower downsampling, concatenation (merged data files) and dimensionality applications which can be constructed into your software program for an intuitive, streamlined data Examination expertise.
"An image just isn't quite worth 1000 dots, nevertheless it seriously helps to see photographs. It is excellent to view illustrations or photos that correlate with staining. For instance, AnnexinV-constructive cells which might be morphologically distinctive than healthy cells, or CD14+ monocytes which can be larger and even more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it can be illuminating to check out the number of doublets slip through standard singlet gates, and terrific to confirm the position of unanticipated double-beneficial situations in my Examination."
Optimizing gating technique. Even sturdy guide singlet gating is mistake-inclined and continues to be a subjective decision place in Practically all flow cytometry assays. Imaging may be used to confirm and modify gates to include only single cells of curiosity.
Dependable picture quality even at high throughput Acoustic focusing and a superior-speed digicam Blend to picture these T cells persistently at minimal or higher flow premiums. Quickly regulate emphasis and digicam configurations to meet experimental needs.
The Attune NxT cytometer may be the dependable workhorse instrument your lab can rely upon for dependable and trustworthy functionality. The Attune CytPix model contains a substantial-pace brightfield digicam and AI-driven graphic Examination software package, with latest updates such as device Finding out tools and an intuitive consumer interface, enabling Increased info precision, accuracy, and faster insights into more info sample biology.
Buyers can see event visuals in serious-time to confirm sample high-quality, enhance protocols, and file the morphology of every cell population recognized for Investigation. Imaging seize also Positive aspects from acoustic focusing, which really helps to place the cells to make sure that a sharp, centered image is received.
AI-pushed automated impression Assessment Together with the Attune Cytometric Application interprets celebration functions into distinctive morphology parameters that may be combined with typical fluorescence and scatter parameters to expose hugely reproducible population variations and exceptional subsets.
Application Notice: Automatic graphic Evaluation decreases consumer-to-user variability in flow cytometry gating techniques
Crafted-in dimensionality reduction instruments greater resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm expected populace resolution in lysed total human blood. User can view occasions from Just about every cluster for Visible confirmation of mobile phenotypes.
No wash/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-previous blood by dilution in 1 mM EDTA ( Qualified UX/UI permits customers to educate AI versions for expanded programs. (A) The “Practice Design” characteristic presents annotation equipment and serious-time model instruction accuracy.
Gating on these extended parameters permits you to swiftly and properly identify populations of curiosity and make sure gating approach with little if any manual overview. The Attune software program features use of condition-of-the-art machine Studying Investigation applications in just an expertly developed consumer interface, which makes it exceptionally simple to operate.
Since the cell line experienced Earlier been analyzed on the Attune CytPix Flow Cytometer, the researchers went again to the photographs and had been in a position to doc the microbial an infection a minimum of five days before.
Agilent Benefit Guarantee guarantees you no less than a decade of instrument use in the day you buy. Usually, We'll credit rating you with the residual price of the process towards an upgraded product.
What gave the impression to be a homogeneous inhabitants is in fact much more various—an insight that should be thought of when interpreting results.